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saci hf restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs saci hf restriction enzyme
    Saci Hf Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/saci+hf+restriction+enzyme/SacI-HF/pm41760616-236-5-11
    Average 96 stars, based on 1103 article reviews
    saci hf restriction enzyme - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.
    Article Snippet: The pGEM-T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM-T Easy vector according to the manufacturer’s instructions and subsequently linearised using the SacI-HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Article Title: [2305.05075] Synthesis of Length-Tunable DNA Carriers for Nanopore Sensing
    Article Snippet: Recover DNA product with spin column (e.g. PureLink PCR Purification Kit, Invitrogen) per manufacturer’s instructions SI for “Synthesis of Length-Tunable DNA Carriers for Nanopore Sensing” by Roelen et al. 3/9 .. Combine on ice: - 16 μL PCR product (~500 ng/μL, from reaction A) - 2 μL KpnI-HF restriction enzyme (20 U/μL, NEB) - 2 μL SacI-HF restriction enzyme (20 U/μL, NEB) - 10 μL CutSmart reaction buffer (10×, NEB) - 70 μL nuclease-free water (Invitrogen) 2. ..

    Article Title: Synthesis of length-tunable DNA carriers for nanopore sensing
    Article Snippet: Recover DNA product with spin column (e.g. PureLink PCR Purification Kit, Invitrogen) per manufacturer’s instructions B) Double Restriction Digest 1. .. Combine on ice: - 16 μL PCR product (~500 ng/μL, from reaction A) - 2 μL KpnI-HF restriction enzyme (20 U/μL, NEB) - 2 μL SacI-HF restriction enzyme (20 U/μL, NEB) - 10 μL CutSmart reaction buffer (10×, NEB) - 70 μL nuclease-free water (Invitrogen) 2. ..

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR‐Based Quantification of Virus Genomes
    Article Snippet: The pGEM‐T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM‐T Easy vector according to the manufacturer's instructions and subsequently linearised using the SacI‐HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Clone Assay:

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.
    Article Snippet: The pGEM-T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM-T Easy vector according to the manufacturer’s instructions and subsequently linearised using the SacI-HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR‐Based Quantification of Virus Genomes
    Article Snippet: The pGEM‐T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM‐T Easy vector according to the manufacturer's instructions and subsequently linearised using the SacI‐HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Plasmid Preparation:

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR-Based Quantification of Virus Genomes.
    Article Snippet: The pGEM-T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM-T Easy vector according to the manufacturer’s instructions and subsequently linearised using the SacI-HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.

    Article Title: Comparative Assessment of Different Nucleic Acid Templates for Use as Standards in the qPCR‐Based Quantification of Virus Genomes
    Article Snippet: The pGEM‐T Easy Vector System (Promega, Cat No: A1380) was then used for the generation of plasmid standards. .. Each PCR product was cloned into the pGEM‐T Easy vector according to the manufacturer's instructions and subsequently linearised using the SacI‐HF restriction enzyme (NEB, Cat no: R3156S). .. Purification of all DNA standards was performed using the GeneJET Gel Extraction Kit (Thermo Fisher Scientific, Cat no: K0691) from a 1% agarose gel containing 0.5 μg/mL ethidium bromide.

    Recombinant:

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.

    Control:

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.

    Transformation Assay:

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.

    Yeast:

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.

    Expressing:

    Article Title: Novel approach for untreated household PET waste depolymerization: recombinant extracellular thermostable hydrolases.
    Article Snippet: Plastics represent a global environmental challenge due to their widespread use and persistent accumulation.. This study is focused on the enzymatic depolymerization of non-pretreated household PET waste using two plastic-degrading esterases, LCC and IsPETase, leveraging the advantages of the extracellular expression in a Pichia pastoris system.. The simple recovery and improved stability enabled by this expression system are crucial foundations in the development of biocatalytic remediation processes.



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